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Acceptable Tube Types
- Purple top tube (EDTA anti-coagulant). Commonly 4.0 or 10mL volume tubes for collection of whole blood.
- Green top tube (lithium heparin anti-coagulant). Commonly 4.0, 5.0 or 10mL tube.
- Green top tube with serum separator gel (lithium heparin anti-coagulant). Commonly 5.0 or 10.0mL volume.
- Blue top tube (sodium citrate anti-coagulant. Commonly 3.0, 5.0 or 10mL tubes for collection of whole blood. Used primarily for coagulation testing.
- Yellow top tube (ACD, acid-citrate-dextrose anti-coagulant). Uncommon sample, but often received as 10mL tube.
Variations (added per request to study protocol).
- Spin at 3000rpm for 10 minutes at room temperature (25*C).
- Use a sterile pipette to transfer plasma to a pour-off tube. Do not contact the buffy-coat layer overlying the packed red cells.
- Cap the aliquot tube, record sample/volume in Crimson application and label.
- Spin time: Can be modified from 10 minutes.
- Spin temperature: Can be modified from 25*C (4*C-42*C).
- Storage conditions: ambient, 4*C, -20*C, -80*C.
- Aliquotting: Can request >1 aliquot in other tube type. Available tube types include the following:
- Eppendorf tubes: 0.6mL, 1.8mL, slim tube.
- Screw capped tubes: 0.6mL, 2.0mL, 10mL.
- Cryovials: specify volume, size (1″, 2″).