Serum Processing

Acceptable Tube Types
  • Red top tube (no anti-coagulant). Commonly 5.0 or 10mL volume tubes for collection of whole blood.
  • Tiger top tube with serum separator gel. Commonly 10mL volume tube for collection of whole blood.
  • Gold top tube with thrombin pro-coagulant. 2.5, 3.5 and 5.0 mL tubes for collection of whole blood.
  • Gold top tube with thrombin pro-coagulant and serum separator gel. 2.5, 3.5 and 5.0mL tube volumes for collection of whole blood.
Standard Procedure
  1. Spin at 3000rpm for 10 minutes at room temperature (25*C).
  2. Use a sterile pipette to transfer serum to a serum aliquot tube.
  3. Cap the aliquot tube, record sample/volume in Crimson application and label.
Variations (added per request to study protocol).
  • Spin time: Can be modified from 10 minutes.
  • Spin temperature: Can be modified from 25*C (4*C-42*C).
  • Storage conditions: ambient, 4*C, -20*C, -80*C.
  • Aliquotting: Can request >1 aliquot in other tube type. Available tube types include the following:
    • Eppendorf tubes: 0.6mL, 1.8mL, slim tube.
    • Screw capped tubes: 0.6mL, 2.0mL, 10mL.
    • Cryovials: specify volume, size (1", 2").